detection of il-20r1 and il-20r2 mrna in c57bl/6 mice astroglial cells and brain cortex following lps stimulation
نویسندگان
چکیده
background: astrocytes, which comprise ~90% of overall brain mass, are involved in brain immunity. these cells represent the non-professional class of cns-resident apcs and may promote or inhibit cns inflammation depending on the cytokines they secrete. il-10 family of cytokines and their receptors, il-20r1 and il-20r2, may have a role in shifting astrocytes to a neuroprotective or neurodegenerative function. objective: to address the expression of il-20r1 and il-20r2 cytokine receptors in astrocytes and brain cortex of c57bl/6 mice. methods: we investigated the expression of il-20r1 and il-20r2 in c57bl/6 mice astroglial cells and brain cortex in response to lipopolysaccharide (lps), using reverse-transcription polymerase chain reaction (rtpcr) method. results: astrocytes were able to express il-20r1 and il-20r2 mrna not only in response to lps stimulation but also in the absence of lps. furthermore, we found the expression of il-20r1 and il-20r2 mrna in the cortex of adult c57bl/6 mice. conclusions: il-20r1 and il-20r2 are constitutively expressed in the brain. since most neuropathological processes involve astrocytes and inflammatory cytokines, these findings have important implications for future therapeutic strategies.
منابع مشابه
Detection of IL-20R1 and IL-20R2 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex Following LPS Stimulation
Background: Astrocytes, which comprise ~90% of overall brain mass, are involved in brain immunity. These cells represent the non-professional class of CNS-resident APCs and may promote or inhibit CNS inflammation depending on the cytokines they secrete. IL-10 family of cytokines and their receptors, IL-20R1 and IL-20R2, may have a role in shifting astrocytes to a neuroprotective or neurodegener...
متن کاملDetection of IL-20R1 and IL-20R2 mRNA in C57BL/6 mice astroglial cells and brain cortex following LPS stimulation.
BACKGROUND Astrocytes, which comprise ~90% of overall brain mass, are involved in brain immunity. These cells represent the non-professional class of CNS-resident APCs and may promote or inhibit CNS inflammation depending on the cytokines they secrete. IL-10 family of cytokines and their receptors, IL-20R1 and IL-20R2, may have a role in shifting astrocytes to a neuroprotective or neurodegenera...
متن کاملstudy of the expressions of il-20r1 and il-20r2 in c57bl/6 mice astroglial cells
objective: astrocytes are the most abundant glial cell type. they may promote or inhibit cns inflammation depending on which cytokines are secreted. astrocytes also have immune roles. il-19, il-20, and il-24 activate a heterodimer receptor composed of the il-20r1 α-chain and the il-20r2 β-chain. it has long been considered that signaling by these receptor complexes affects immunological reactio...
متن کاملDetection of Interleukin-19 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex
Introduction: Astrocytes are the most abundant glial cell type. In addition to their neurological roles, astrocytes also have immune functions. They have been involved in antigen presentation in the central nervous system (CNS). Activated astrocytes express adhesion molecules, chemokines and release several inflammatory mediators, pro-inflammatory cytokines, neurotrophic and neuroprotective fac...
متن کاملdetection of interleukin-19 mrna in c57bl/6 mice astroglial cells and brain cortex
introduction: astrocytes are the most abundant glial cell type. in addition to their neurological roles, astrocytes also have immune functions. they have been involved in antigen presentation in the central nervous system (cns). activated astrocytes express adhesion molecules, chemokines and release several inflammatory mediators, pro-inflammatory cytokines, neurotrophic and neuroprotective fac...
متن کاملInterleukin 24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2.
Interleukin 24 (IL-24) encodes a secreted protein that exhibits significant homology to the interleukin 10 (IL-10) family of cytokines. Here we show that the human IL-24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. The latter is also the receptor for IL-20. COS cells transfected with either IL...
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عنوان ژورنال:
iranian journal of immunologyجلد ۱۰، شماره ۲، صفحات ۶۲-۶۹
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